蛋白質體學

Proteomics Last modified: 2007/01/15

	Please review the life cycle of S. cerevisiae, Yeast two-hybrid assay
	Reading assignment (Nature 1999)

	Reading assignment for everyone: 書上 6.3 的部份
	第一組 Yeast two-hybrid (Nature 2000, 403: 623-627),
	第二組 TAP-tag + MS (Nature 2002, 415, 141-147)
	第三組 FLAG-tag + MS (Nature 2002, 415, 180-183
	我 Pooling strategy (Science 1999, 286,1153-1155

3/17 Principle and application of Mass Spectroscopy (胡安仁老師)

3/24 Protein Chip (陳怡成老師)

3/31 Structural Proteomics: X-ray crystallography and NMR spectroscopy (陳怡成老師)

4/7 Other structural techniques

	Suggested reading: From words to literture in structural proteomics (Nature 2003, 422, 216-225)
	Slides on EM (pdf file)
	Slides on myosin motor (pdf file)

5/12 Summary (pdf file)

分組學期報告:

What's wrong with my child? (in pdf format)

	江承堯 What is dystrophy?
	陳榮傑 How do you know if you have dystrophy or not?
	王志傑 How does dystrophin work?
	黃威靈 Can we prevent dystrophy?
	許魏瀚 The future of dystrophy

Why can't I just take a pill to lose weight? (in pdf format)

	李蕙芳 What is leptin?
	蔡宗穆 How does leptin regulate weight homeostasis ?
	黃永睿 Why can leptin work in mice?
	袁忠豪 Why can't leptin work in human?
	侯凱中 Some other proteins about weight control
	鄭文豪 Other methods about losing weight

Why can't we cure more diseases? (in pdf format)

References:

a. Random transposon tagging strategy (p. 162-166, Question 1-18)

Application in yeast proteome (Nature 1999)

	The multipurpose transposon system (PNAS 1997)
	Clustering methods (PNAS 1998)

b. Bar code (UPTAG and DOWNTAG) (p. 166-170, Question 19-25)

Application in yeast proteome (Science 1999)

c. RNAi (p. 170-171)

	Background (Fire, Nature 1998, News and Views)
	Application in C. elegans chromosome I (Nature 2000)
	Application in C. elegans chromosome III (Nature 2000)

Protein-protein interactions

Data integration

	in silico (Cell 2000)
	Integrated circuit (Science 2002)

More to come...