蛋白質體學

Proteomics Last modified: 2007/01/15

3/10 slide (in pdf format)

Random transposon tagging strategy

Application in yeast proteome (Nature 1999)

	The multipurpose transposon system (PNAS 1997)
	Clustering methods (PNAS 1998)

3/17 slide (in pdf format)

Bar code (UPTAG and DOWNTAG)

Application in yeast proteome (Science 1999)

RNAi

Background (Review)

	Application in C. elegans chromosome I (Nature 2000)
	Application in C. elegans chromosome III (Nature 2000)

3/24 slide (in pdf format)

Protein-protein interaction

Yeast two-hybrid (Nature 2000, 403: 623-627)

Basic principle of Mass Spectrometry

	TAP-tag + MS (Nature 2002, 415, 141-147)
	FLAG-tag + MS (Nature 2002, 415, 180-183)

Pooling strategy (Science 1999, 286, 1153-1155)

Protein chip (Science 2000, 289, 1760-1763)

3/31/2005

Case study: strategy for protein-protein interaction

Induced proteolysis of yeast DNA replication proteins (Nature 2003, 423, 720-724)

鄧文豪 pdf file

msSUS for membrane proteins (PNAS 2004, 101, 12242-12247)

張淳淳 pdf file

Leuciferase complementation in cells and living animals (PNAS 2004, 101, 12288-12293)

簡禎瑩 pdf file

Luminescence-based mammalian interactome mapping (LUMIER) (Science 2005, 307, 1621-1625)

許禮汎 pdf file

TAP for E. coli interactome (Nature 2005, 433, 531-537)

葉明鑫 pdf file

References:

Data integration

	in silico (Cell 2000)
	Integrated circuit (Science 2002)

More to come...